NEW SIMPLIFIED PROCEDURES FOR THE EXTRACTION AND SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF RETINOL, TOCOPHEROLS AND CAROTENOIDS IN HUMAN SERUM

被引：100

作者：

BARUA, AB

KOSTIC, D

OLSON, JA

机构：

[1] Department of Biochemistry and Biophysics, Iowa State University, Ames

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 617卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80496-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A short, simple extraction procedure and a sensitive reversed-phase high-performance liquid chromatographic assay, which utilizes isocratic elution and detection either by a photodiode-array detector or by two detectors set at 300 and 450 nm, have been developed to measure retinol, tocopherols and several carotenoids in human serum simultaneously. By relying on characteristic UV-visible spectra, seventeen carotenoids, retinol and alpha- and gamma-tocopherols were identified in concentrated serum extracts by use of the three-dimensional data mode of a photodiode-array detector. The presence of some recently reported carotenoids in human serum has been confirmed.

引用

页码：257 / 264

页数：8

共 27 条

[1] SIMULTANEOUS ANALYSIS OF INDIVIDUAL CAROTENOIDS, RETINOL, RETINYL ESTERS, AND TOCOPHEROLS IN SERUM BY ISOCRATIC NONAQUEOUS REVERSED-PHASE HPLC [J].