MULTIFUNCTIONAL APPARATUS FOR ELECTROKINETIC PROCESSING OF PROTEINS

被引：54

作者：

HORVATH, ZS

CORTHALS, GL

WRIGLEY, CW

MARGOLIS, J

机构：

[1] GRADIPORE,SYDNEY,NSW,AUSTRALIA

[2] UNIV TECHNOL SYDNEY,SYDNEY 2007,NSW,AUSTRALIA

[3] CSIRO,DIV PLANT IND,GRAIN QUAL RES LAB,N RYDE,NSW 2113,AUSTRALIA

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 07期

关键词：

D O I：

10.1002/elps.11501501141

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

New equipment (the ''Gradiflow(TM)'') has been designed and constructed to provide efficient large-scale preparative fractionation of macromolecules, based on charge and/or size differences, as well as the concentration of macromolecules and electrodialysis. Examples of its capability are the separation of a mixture of haemoglobin (50 mg) from bovine serum albumin (50 mg) within 15 min (based on charge differences at pH 6.8), the purification of phycoerythrin from a crude extract on the basis of size, and the fractionation of serum proteins into two discrete size classes.

引用

页码：968 / 971

页数：4

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