LIPID METABOLISM IN KIDNEY AND LIVER TISSUE FROM NORMAL AND DIABETIC RATS

1. 1. Normal and diabetic rats were fed four different diets: laboratory chow, 5% corn oil, 5% hydrogenated coconut oil and a fat-deficient diet. Diabetic liver slices show decreased O2 utilization. The diabetic kidney tissue responded with increased O2 consumption. [1-14C]acetate labeling of fatty acids was depressed in all the diabetic livers. Diabetic kidney tissue had higher rates of lipogenesis than control kidney tissue. Cholesterol synthesis from acetate was depressed in the diabetic liver except when the diet fed was fat deficient. Diabetes did not influence cholesterol labeling in kidney tissue. 2. 2. Liver from normal and diabetic rats fed hydrogenated coconut oil and fatdeficient diets had increased amounts of monoenoic acids compared to corn-oil-fed animals. Normal liver from animals fed saturated-fat and fat-deficient diets had decreased amounts of stearic and linoleic acid. The diabetic livers had more linoleic acid than normal livers. Kidney tissue from normal animals fed the saturated-fat and fat-deficient diets also had increased amounts of oleic acid and decreased amounts of linoleic acid compared to that from animals fed unsaturated fatty acid. Kidney tissue from diabetic animals had greater amounts of linoleic acid. © 1969.