STRUCTURAL-ANALYSIS OF THE HEPTOSE/HEXOSE REGION OF THE LIPO-POLYSACCHARIDE FROM ESCHERICHIA-COLI K-12 STRAIN W3100

被引：48

作者：

HOLST, O ^{[1
]}

ZAHRINGER, U ^{[1
]}

BRADE, H ^{[1
]}

ZAMOJSKI, A ^{[1
]}

机构：

[1] POLISH ACAD SCI, INST ORGAN CHEM, PL-01224 WARSAW, POLAND

来源：

CARBOHYDRATE RESEARCH | 1991年 / 215卷 / 02期

关键词：

D O I：

10.1016/0008-6215(91)84031-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The disaccharide L-glycero-D-manno-heptosyl-D-glucose was isolated from the lipopolysaccharide (LPS) of Escherichia coli K-12 strain W3100 after partial hydrolysis with acid, and the structure was determined by methylation analysis, n.m.r. spectroscopy, and comparison with a synthetic standard. In addition, the oligosaccharides L,D-Hep-D-Glc-D-Glc and L,D-Hep-D-Glc-D-Glc-D-Glc were isolated, and their structures were established by g.l.c.-m.s. and methylation analysis. The results indicated that L-glycero-D-manno-heptose, a characteristic constituent of the inner core region, may also occur in the outer core region which, in E. coli, is generally composed of hexoses. A revised structure of the carbohydrate backbone of the hexose/heptose region of the LPS is given.

引用

页码：323 / 335

页数：13

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