THE ANALYSIS OF CLOBAZAM AND ITS METABOLITE DESMETHYLCLOBAZAM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：15

作者：

STREETE, JM

BERRY, DJ

NEWBERY, JE

机构：

[1] THAMES POLYTECH,POISONS UNIT,AVONLEY RD,LONDON SE14 5ER,ENGLAND

[2] THAMES POLYTECH,SCH BIOL & CHEM SCI,LONDON SE14 5ER,ENGLAND

来源：

THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 04期

关键词：

CLOBAZAM; DESMETHYLCLOBAZAM; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1097/00007691-199107000-00010

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The analysis of clobazam and its metabolite desmethylclobazam by high-performance liquid chromatography is described. After adding an internal standard 500-mu-l of plasma is extracted under basic conditions into dichloroethane. The organic solvent is then evaporated to dryness and the residue reconstituted in 100-mu-l of mobile phase prior to injecting an aliquot (30-mu-l) onto a Hypersil 5 MOS column, which is eluted with acetonitrile/acetate buffer (pH 5.4) 40:60 vol/vol. The components are separated in approximately 12 min. Using this method, 15-mu-g L-1 of clobazam and 30-mu-g L-1 of desmethylclobazam can be detected. The method is suitable for the therapeutic monitoring of these two drugs in patient samples.

引用

页码：339 / 344

页数：6

共 16 条

[1] MICRODETERMINATION OF CLOBAZAM AND N-DESMETHYLCLOBAZAM IN PLASMA OR SERUM BY ELECTRON-CAPTURE GAS-CHROMATOGRAPHY [J].