SORPTION OF HEAVY-METALS TO THE FILAMENTOUS BACTERIUM THIOTHRIX STRAIN A1

被引:75
作者
SHUTTLEWORTH, KL
UNZ, RF
机构
[1] PENN STATE UNIV,ENVIRONM MICROBIOL LAB,INTERCOLLEGIATE PROGRAM ECOL,UNIV PK,PA 16802
[2] PENN STATE UNIV,DEPT CIVIL & ENVIRONM ENGN,UNIV PK,PA 16802
关键词
D O I
10.1128/AEM.59.5.1274-1282.1993
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A study was undertaken to determine the ability of the filamentous bacterium Thiothrix strain Al to sorb heavy metals from solution. Cells of Thiothrix strain Al were harvested, washed, and suspended in solutions of metals. After an equilibration period, biomass was separated from solution and the metal content in acid-digested cells and/or filtrates was determined by atomic absorption spectrophotometry. Sorption of nickel and zinc was very rapid; most of the sorbed metal was bound in less than 10 min. The sorption data for copper fit the Freundlich isotherm, and nickel and zinc data fit biphasic Freundlich isotherms. Sorption of both nickel and zinc was dependent on cell age. Cells harvested 24 h after inoculation sorbed approximately one-half of the amount of metal per gram cell protein than did cells harvested after 48, 72, or 96 h. Calcium and magnesium effectively competed with zinc for binding sites, whereas potassium had only a slight effect on the capacity of cells to sorb zinc. The primary mechanism of metal sorption apparently was ion exchange, because 66 to 75% of nickel or zinc could be desorbed by placing metal-laden cells in a solution of 5 mM CaCl2. A competition experiment with nickel and zinc indicated that both metals occupied the same sorption sites. The strong chelating agents EDTA and NTA effectively prevented metal uptake, but lactate enhanced the uptake of nickel. Thiothrix strain Al grown in nickel-containing medium had a relatively low uptake of nickel compared with uptake by resting cells suspended in a simple buffer solution.
引用
收藏
页码:1274 / 1282
页数:9
相关论文
共 38 条
[1]  
[Anonymous], [No title captured]
[2]   ROLE OF CELLULAR DESIGN IN BACTERIAL METAL ACCUMULATION AND MINERALIZATION [J].
BEVERIDGE, TJ .
ANNUAL REVIEW OF MICROBIOLOGY, 1989, 43 :147-171
[3]   ENERGY-DEPENDENT ZINC TRANSPORT BY ESCHERICHIA-COLI [J].
BUCHEDER, F ;
BRODA, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 45 (02) :555-559
[4]   DISTRIBUTION OF IRON IN SPHAEROTILUS AND THE ASSOCIATED INHIBITION [J].
CHANG, Y ;
PFEFFER, JT ;
CHIAN, ESK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1980, 40 (06) :1049-1052
[5]  
DOWD JE, 1965, J BIOL CHEM, V240, P863
[6]   CHEMICAL BASIS FOR SELECTIVITY OF METAL-IONS BY THE BACILLUS-SUBTILIS CELL-WALL [J].
DOYLE, RJ ;
MATTHEWS, TH ;
STREIPS, UN .
JOURNAL OF BACTERIOLOGY, 1980, 143 (01) :471-480
[7]   INFLUENCE OF SOLUTION COMPOSITION ON SORPTION OF ZINC BY SOILS [J].
ELRASHIDI, MA ;
OCONNOR, GA .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1982, 46 (06) :1153-1158
[8]   BINDING OF DISSOLVED STRONTIUM BY MICROCOCCUS-LUTEUS [J].
FAISON, BD ;
CANCEL, CA ;
LEWIS, SN ;
ADLER, HI .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (12) :3649-3656
[9]  
Gadd G. M., 1988, Biotechnology Volume 6b. Special Microbial Processes., P401
[10]   HYDROGEN ION BUFFERS FOR BIOLOGICAL RESEARCH [J].
GOOD, NE ;
WINGET, GD ;
WINTER, W ;
CONNOLLY, TN ;
IZAWA, S ;
SINGH, RMM .
BIOCHEMISTRY, 1966, 5 (02) :467-&