MITOCHONDRIAL RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN WILD PHASEOLUS-VULGARIS L - INSIGHTS ON THE DOMESTICATION OF THE COMMON BEAN

被引:45
作者
KHAIRALLAH, MM
SEARS, BB
ADAMS, MW
机构
[1] MICHIGAN STATE UNIV, DEPT CROP & SOIL SCI, E LANSING, MI 48824 USA
[2] MICHIGAN STATE UNIV, DEPT BOT & PLANT PATHOL, E LANSING, MI 48824 USA
关键词
MITOCHONDRIAL RFLPS; COMMON BEAN; CROP DOMESTICATION; FOUNDER EFFECT; GENE POOLS;
D O I
10.1007/BF00227404
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Previous examination of intraspecific mitochondrial DNA (mtDNA) diversity in common bean, Phaseolus vulgaris, showed that five restriction fragment length polymorphisms (RFLPs) distinguish the mitochondrial genomes of the two major gene pools of cultivated beans, the Mesoamerican and the Andean. In the study presented here, mtDNA was used to compare the amount of diversity in cultivated beans to that in collections of wild beans to pin an understanding of how and when the mitochondrial genomes of the gene pools became distinct. The mtDNA of six wild bean accessions from Central and South America were digested with nine restriction endonucleases and analyzed by Southern hybridization. A total of twenty RFLPs were detected demonstrating a significantly higher amount of mtDNA variability in wild beans than in cultivated ones. All of the wild beans had the same mtDNA pattern for four out of the five inter-gene pool RFLPs, indicating that the polymorphism arose soon after domestication: two in the gene pool of the cultivated Mesoamerican beans and two in the gene pool of the cultivated Andean beans. The fifth RFLP must have occurred before domestication since the locus was also polymorphic in the wild beans. Wild beans from the south Andes were distinct and less variable than wild accessions of the north Andes and Mesoamerica. The distribution of mtDNA RFLPs among the wild beans supports the concept of two distinct domestication events for P. vulgaris.
引用
收藏
页码:915 / 922
页数:8
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