MOLECULAR ANALYSIS OF THE NEUROSPORA CLOCK - CLONING AND CHARACTERIZATION OF THE FREQUENCY AND PERIOD-4 GENES

被引：8

作者：

ARONSON, BD

JOHNSON, KA

LIU, QY

DUNLAP, JC

机构：

[1] Department of Biochemistry, Dartmouth Medical School, Hanover, NH

来源：

CHRONOBIOLOGY INTERNATIONAL | 1992年 / 9卷 / 03期

关键词：

NEUROSPORA-CRASSA; FRQ GENE; PRD-4; GENE; GENETIC ANALYSIS;

D O I：

10.3109/07420529209064532

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetic analysis of Neurospora crassa has identified many mutants that affect the biological clock. In this article we review the cloning of two of these genes, frq and prd-4. Both genes were isolated using a chromosome walk technique. Subcloning experiments and subsequent Northern analysis of frq implicate the importance of two transcripts that emanate from this locus. In preliminary data, no protein-coding region is evident in the smaller transcript; the larger transcript contains a 962-amino acid open reading frame. The open reading frame shows limited homology to per, a clock gene identified in Drosophila. Sequence analysis of all existing frq alleles suggests that the defect in each case lies within the open reading frame. Successful cloning of the prd-4 gene required walking a distance of > 40 kb. A physical map of this region has been constructed using restriction analysis. The dominance-recessive relationship of prd-4 and prd-4+ was established by examining the period lengths of strains harboring a wide range of prd-4/prd-4+ nuclear ratios.

引用

页码：231 / 239

页数：9

共 20 条

[1]

Konopka R.J., Benzer S., Clock mutants of Drosophila melanogaster, Proc Natl Acad Sci USA, 68, pp. 2112-2116, (1971)

[2]

Feldman J.F., Genetic approaches to circadian clocks, Anna Rev Plant Physiol, 33, pp. 583-608, (1982)

[3]

Bruce V.G., Mutants of the biological clock in Chlamydomonas reinhhardi, Genetics, 70, pp. 537-548, (1972)

[4]

Dunlap J.C., Closely watched clocks: molecular analysis of circadian rhythms in Nenrospora and Drosophila, Trends Genet, 6, pp. 159-165, (1990)

[5]

Gardner G.F., Feldman J.F., Temperature compensation of circadian periodicity in clock mutants of Neurospora crassa, Plant Physiol, 68, pp. 1244-1248, (1981)

[6]

Hall J., Genetics of circadian rhythms, Annu Rev Genet, 24, pp. 659-697, (1991)

[7]

Feldman J.F., Hoyle M., Isolation of circadian clock mutants of Neurospora crassa, Genetics, 75, pp. 605-613, (1973)

[8]

Feldman J.F., Hoyle M., Complementation analysis of linked circadian clock mutants of Neurospora crassa, Genetics, 82, pp. 9-17, (1976)

[9]

Loros J.J., Richman A., Feldman J.F., A recessive circadian clock mutant at the frq locus in Neurospora crassa, Genetics, 114, pp. 1095-1110, (1986)

[10]

Gardner G.F., Feldman J.F., The frq locus in Neurospora crassa: a key element in circadian clock organization, Genetics, 96, pp. 877-886, (1980)

← 1 2 →