ELECTROPHYSIOLOGICAL EFFECTS OF TACHYKININS AND CAPSAICIN ON GUINEA-PIG BRONCHIAL PARASYMPATHETIC GANGLION NEURONS

被引:99
作者
MYERS, AC
UNDEM, BJ
机构
[1] Allergy Center, Baltimore, Maryland
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1993年 / 470卷
关键词
D O I
10.1113/jphysiol.1993.sp019881
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. We evaluated the effects of neurokinins, tachykinin analogues, or capsaicin on passive membrane properties of guinea-pig bronchial parasympathetic neurones using intracellular recording techniques. 2. Substance P (SP) and the tachykinin analogue, acetyl-[Arg6,Sar9,Met(O2)11]-SP(6-11) (ASMSP), at concentrations selective for the neurokinin (NK)-1 receptor subtype, depolarized the resting potential (3 and 5 mV, respectively) with no change in input resistance. Neurokinin A and betaAla8NKA(4-10), at concentrations selective for the NK-2 receptor subtype (0.1 mum), were without effect. 3. Neurokinin B (NKB) and [Asp5,6, methyl-Phe8]SP(5-11) (senktide analogue), at concentrations selective for NK-3 receptor subtype, elicited maximum depolarizations of 16 +/- 2 mV for both agonists. The peak of the depolarization was associated with an decrease in membrane resistance (35 +/- 4 and 50 +/- 7 % respectively). 4. Capsaicin (1 mum) elicited a 3-24 mV depolarization of the resting potential of thirteen of eighteen bronchial ganglion neurones and decreased the input resistance of seven of thirteen of these neurones. The effects of capsaicin were reduced by desensitization with senktide analogue at a concentration selective for the NK-3 receptor subtype, whereas a non-peptide NK-1 receptor antagonist had no effect. 5. Using voltage clamp analysis, capsaicin and senktide analogue evoked an inward current and an increase in membrane conductance at the resting membrane potential. The reversal potential for senktide analogue was estimated to be + 4 mV. 6. These data support the hypothesis that neurokinin-containing nerve terminals are localized within guinea-pig bronchial parasympathetic ganglia and, when released, the predominant effect of the neurokinins is by activation of NK-3 receptors.
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页码:665 / 679
页数:15
相关论文
共 37 条
[1]   SUBSTANCE-P INHIBITS THE M-CURRENT IN BULLFROG SYMPATHETIC NEURONS [J].
ADAMS, PR ;
BROWN, DA ;
JONES, SW .
BRITISH JOURNAL OF PHARMACOLOGY, 1983, 79 (02) :330-333
[2]  
AIZAWA H, 1990, RESPIRATION, V57, P388
[3]  
ARENAS E, 1991, J NEUROSCI, V11, P2332
[4]   CAPSAICIN CAUSES PROLONGED INHIBITION OF VOLTAGE-ACTIVATED CALCIUM CURRENTS IN ADULT-RAT DORSAL-ROOT GANGLION NEURONS IN CULTURE [J].
DOCHERTY, RJ ;
ROBERTSON, B ;
BEVAN, S .
NEUROSCIENCE, 1991, 40 (02) :513-521
[5]   EFFECTS OF SUBSTANCE-P ON NEURONS OF THE INFERIOR MESENTERIC GANGLIA OF THE GUINEA-PIG [J].
DUN, NJ ;
MINOTA, S .
JOURNAL OF PHYSIOLOGY-LONDON, 1981, 321 (DEC) :259-271
[6]  
FOREMAN JC, 1983, AGENTS ACTIONS, V13, P233
[7]   NEUROKININ3-RECEPTORS ARE LINKED TO INOSITOL PHOSPHOLIPID HYDROLYSIS IN THE GUINEA-PIG ILEUM LONGITUDINAL MUSCLE MYENTERIC PLEXUS PREPARATION [J].
GUARD, S ;
WATLING, KJ ;
WATSON, SP .
BRITISH JOURNAL OF PHARMACOLOGY, 1988, 94 (01) :148-154
[8]   FACILITATION BY TACHYKININS OF NEUROTRANSMISSION IN GUINEA-PIG PULMONARY PARASYMPATHETIC NERVES [J].
HALL, AK ;
BARNES, PJ ;
MELDRUM, LA ;
MACLAGAN, J .
BRITISH JOURNAL OF PHARMACOLOGY, 1989, 97 (01) :274-280
[9]  
JANSCO G, 1985, NEUROSCI LETT, V59, P209
[10]   THE MECHANISM OF TACHYKININ-INDUCED BRONCHOCONSTRICTION IN THE RAT [J].
JOOS, GF ;
PAUWELS, RA ;
VANDERSTRAETEN, ME .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1988, 137 (05) :1038-1044