RANDOMLY DISTRIBUTED DNA DOUBLE-STRAND BREAKS AS MEASURED BY PULSED-FIELD GEL-ELECTROPHORESIS - A SERIES OF EXPLANATORY CALCULATIONS

被引：34

作者：

CEDERVALL, B

KALLMAN, P

机构：

[1] Department of Medical Radiation Physics, Division of Tumour Biology, Karolinska Institutet, Stockholm, S-104 01

[2] Department of Medical Radiation Physics, Karolinska Institutet, Stockholm, S-104 01

来源：

RADIATION AND ENVIRONMENTAL BIOPHYSICS | 1994年 / 33卷 / 01期

关键词：

D O I：

10.1007/BF01255270

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this article is to characterize expressions of relevance to the interpretation of pulsed field gel electrophoresis (PFGE) experiments where randomly distributed double-strand breaks (DSBs) are detected as smears of DNA fragments. Specifically, equations for conversion of percentages of fragments in defined size ranges to DSBs were derived. Several models have been used, one of which is based on theoretically fragmented DNA from the fission yeast Schizosaccharomyces pombe, which has three PFGE separable chromosomes.

引用

页码：9 / 21

页数：13

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