ON THE PRESENCE OF PHOSPHORYLATED SPHINGOID BASES IN RAT-TISSUES - A MASS-SPECTROMETRIC APPROACH

被引：28

作者：

VANVELDHOVEN, PP ^{[1
]}

DECEUSTER, P ^{[1
]}

ROZENBERG, R ^{[1
]}

MANNAERTS, GP ^{[1
]}

DEHOFFMANN, E ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN, CICO, B-1348 LOUVAIN, BELGIUM

来源：

FEBS LETTERS | 1994年 / 350卷 / 01期

关键词：

ALIPHATIC AMINE; SIGNAL TRANSDUCTION; SPHINGOSINE KINASE; SPHINGENINE-PHOSPHATE; SPHINGOSINE-PHOSPHATASE; SPHINGOSINE-PHOSPHATE LYASE;

D O I：

10.1016/0014-5793(94)00739-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and straightforward procedure to analyze phosphorylated sphingoid bases has been developed. After phase separation of lipid extracts under alkaline conditions, the compounds were quantitatively recovered in the aqueous upper phase. Following a clean-up of the aqueous phase on C18-solid phase extraction columns, the amino-group of the bases was derivatized by means of phenylisothiocyanate addition. FAB-MS of the phenylthiocarbamate derivatives of sphingenine- and sphinganine-phosphate in the negative mode revealed the expected pseudo-molecular ions (M-1) at 513 m/z and 515 m/z, respectively. Moreover, a typical fragmentation pattern, characterized by the loss of the phenylthiocarbamate moiety (m/z = 135), was observed. When applied to rat tissues, the presence of sphingenine-phosphate in brain, kidney and liver could easily be demonstrated. Highest levels, amounting to 5 nmol/g of wet weight, were present in brain.

引用

页码：91 / 95

页数：5

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