CLONING, SEQUENCING AND EXPRESSION OF PSEUDOMONAS-TESTOSTERONI GENE ENCODING 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE

被引：14

作者：

ABALAIN, JH ^{[1
]}

DISTEFANO, S ^{[1
]}

ABALAINCOLLOC, ML ^{[1
]}

FLOCH, HH ^{[1
]}

机构：

[1] FAC MED BREST,BACTERIOL LAB,F-29200 BREST,FRANCE

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1995年 / 55卷 / 02期

关键词：

D O I：

10.1016/0960-0760(95)00170-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the cloning, sequencing and expression of the 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD) gene of Pseudomonas testosteroni. A genomic library of P. testosteroni total DNA constructed from SauIIIA digests ligated to an lambda gtII vector was probed with a polyclonal antibody raised against purified enzyme. Subclones derived from a recombinant phage containing a 1746 bp insert were sequenced and found to contain an open reading frame of 696 bp that corresponds to a protein of 231 amino acid residues. A search for homologous proteins was performed. No similarity was observed when comparing 3 alpha-HSD with known members of the short-chain dehydrogenase family. However a small proteic fragment (80 amino acids) shows homology with the N-terminal sequence of bacterial L7/L12 ribosomal proteins.

引用

页码：233 / 238

页数：6

共 30 条

[1] CLONING, DNA SEQUENCING AND EXPRESSION OF (3-17)BETA HYDROXYSTEROID DEHYDROGENASE FROM PSEUDOMONAS-TESTOSTERONI [J].