INTERFERENCE OF GOOD BUFFERS AND OTHER BIOLOGICAL BUFFERS WITH PROTEIN DETERMINATION

被引：23

作者：

LLEU, PL

REBEL, G

机构：

[1] Centre de Neurochimie CNRS

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90210-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interference of low concentrations of Hepes and other buffers commonly used in protein determination was studied. The data show that some of these buffers interfere to differing degrees with protein determination according to the Lowry method. A study of the structure-interference relationship suggests that the group ethanolamine is involved in this interference. No interference was observed when protein was measured using bicinchonic acid at the same concentration as the Lowry reagent. © 1991.

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页码：215 / 218

页数：4

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