METHOD TO IDENTIFY GENOMIC TARGETS OF DNA-BINDING PROTEINS

被引：30

作者：

SOMPAYRAC, L

DANNA, KJ

机构：

[1] Department Molecular Biology, University of Colorado, Boulder

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 09期

关键词：

D O I：

10.1073/pnas.87.9.3274

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.

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页码：3274 / 3278

页数：5

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