INVIVO ACCUMULATION OF SULFATED GLYCOPROTEIN-2 MESSENGER-RNA IN RAT THYMOCYTES UPON DEXAMETHASONE-INDUCED CELL-DEATH

被引：91

作者：

BETTUZZI, S ^{[1
]}

TROIANO, L ^{[1
]}

DAVALLI, P ^{[1
]}

TROPEA, F ^{[1
]}

INGLETTI, MC ^{[1
]}

GRASSILLI, E ^{[1
]}

MONTI, D ^{[1
]}

CORTI, A ^{[1
]}

FRANCESCHI, C ^{[1
]}

机构：

[1] UNIV MODENA,IST PATOL GEN,I-41100 MODENA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91637-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two hours after a single intraperitoneal injection of dexamethasone (20 μg/Kg b.w.) into adult male rats, a typical ladder of DNA fragments was detectable upon separation on agarose gels of DNA from thymocytes. This became maximally evident at 4 hours. Accumulation of sulfated glycoprotein-2 (SGP-2) mRNA, whose rate of expression has been associated to the processes of programmed cell death, preceded the appearance of DNA degradation, starting to increase as early as 30 min after steroid injection, and maintained higher than controls until 8 hrs; a different time course was shown by changes in the levels of beta-actin mRNA. In the spleen, under the same conditions, the SGP-2 message also increased at 30 min, prior to DNA fragmentation, but decreased thereafter below the control value. © 1991.

引用

页码：810 / 815

页数：6

共 21 条

[1] COMPARISON OF 3 ACTIN-CODING SEQUENCES IN THE MOUSE - EVOLUTIONARY RELATIONSHIPS BETWEEN THE ACTIN GENES OF WARM-BLOODED VERTEBRATES [J].