POTENTIAL ROLE OF PHOSPHOLIPASE-A2 IN HL-60 CELL-DIFFERENTIATION TO MACROPHAGES INDUCED BY PROTEIN-KINASE-C ACTIVATION

被引：57

作者：

ASAOKA, Y ^{[1
]}

YOSHIDA, K ^{[1
]}

SASAKI, Y ^{[1
]}

NISHIZUKA, Y ^{[1
]}

机构：

[1] KOBE UNIV,SCH MED,DEPT BIOCHEM,KOBE 650,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 11期

关键词：

2-LYSOPHOSPHATIDYLCHOLINE; CIS-UNSATURATED FATTY ACID; PHORBOL ESTER; DIACYLGLYCEROL;

D O I：

10.1073/pnas.90.11.4917

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

2-Lysophosphatidylcholine and cis-unsaturated fatty acids such as linoleic and linolenic acids, which are the products of the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2 (EC 3.1.1.4), significantly potentiate the differentiation of HL-60 cells to macrophages that is induced by either a membrane-permeant diacylglycerol or a phorbol ester. The cell differentiation was assayed by measuring the expression of CD11b, one of the cell surface markers of macrophages, and also by the appearance of phagocytic activity. Snake venom phospholipase A2 added directly to the cells is also active for this potentiation. Neither lysophosphatidylcholine, fatty acid, nor phospholipase A2 is active unless a membrane-permeant diacylglycerol or a phorbol ester is present. The results presented provide further evidence that activation of phospholipase A2 may be intimately related to the signal transduction pathway through protein kinase C.

引用

页码：4917 / 4921

页数：5

共 30 条

[1] SUSTAINED ACTIVATION OF PROTEIN-KINASE-C IS ESSENTIAL TO HL-60 CELL-DIFFERENTIATION TO MACROPHAGE [J].