CLONING, EXPRESSION AND SEQUENCE-ANALYSIS OF CDNA FOR THE CA2+-BINDING PHOTOPROTEIN, MITROCOMIN

被引:55
作者
FAGAN, TF
OHMIYA, Y
BLINKS, JR
INOUYE, S
TSUJI, FI
机构
[1] UNIV CALIF SAN DIEGO, SCRIPPS INST OCEANOG, DIV MARINE BIOL RES 0202, LA JOLLA, CA 92093 USA
[2] OSAKA BIOSCI INST, SUITA, OSAKA 565, JAPAN
[3] UNIV WASHINGTON, FRIDAY HARBOR LABS, FRIDAY HARBOR, WA 98250 USA
来源
FEBS LETTERS | 1993年 / 333卷 / 03期
关键词
BIOLUMINESCENCE; HALISTAURA; CALCIUM; AEQUORIN; CLYTIN; C-TERMINAL TYROSINE;
D O I
10.1016/0014-5793(93)80675-K
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The primary structure of mitrocomin consists of 190 amino acid residues, with three Ca2+-binding sites and a tyrosine residue at tbe C-terminus. Mitrocomin shows an amino acid sequence homology of 67.9% and 60.7% when compared with aequorin and clytin, respectively. The amino acid residues Cys152, His58, His169, Trp12, Trp86, Trp108, Trp129 and Trp173 are conserved in all three photoproteins, suggesting that they play a role in light emission.
引用
收藏
页码:301 / 305
页数:5
相关论文
共 29 条
  • [1] Blinks J.R., 1978, Methods in Enzymology, V57, P292
  • [2] EXTRACTION, PARTIAL-PURIFICATION AND PROPERTIES OF OBELIN, CALCIUM-ACTIVATED LUMINESCENT PROTEIN FROM HYDROID OBELIA-GENICULATA
    CAMPBELL, AK
    [J]. BIOCHEMICAL JOURNAL, 1974, 143 (02) : 411 - 418
  • [3] AMINO-ACID SEQUENCE OF THE CALCIUM-DEPENDENT PHOTOPROTEIN AEQUORIN
    CHARBONNEAU, H
    WALSH, KA
    MCCANN, RO
    PRENDERGAST, FG
    CORMIER, MJ
    VANAMAN, TC
    [J]. BIOCHEMISTRY, 1985, 24 (24) : 6762 - 6771
  • [4] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
  • [5] LUMINESCENCE IN HYDROMEDUSAE
    DAVENPORT, D
    NICOL, JAC
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1955, 144 (916): : 399 - 411
  • [6] DIDEOXY SEQUENCING METHOD USING DENATURED PLASMID TEMPLATES
    HATTORI, M
    SAKAKI, Y
    [J]. ANALYTICAL BIOCHEMISTRY, 1986, 152 (02) : 232 - 238
  • [7] INOUE S, 1975, CHEM LETT, P141, DOI 10.1246/cl.1975.141
  • [8] CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR THE LUMINESCENT PROTEIN AEQUORIN
    INOUYE, S
    NOGUCHI, M
    SAKAKI, Y
    TAKAGI, Y
    MIYATA, T
    IWANAGA, S
    MIYATA, T
    TSUJI, FI
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (10) : 3154 - 3158
  • [9] CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR THE CA-2+-ACTIVATED PHOTOPROTEIN, CLYTIN
    INOUYE, S
    TSUJI, FI
    [J]. FEBS LETTERS, 1993, 315 (03): : 343 - 346
  • [10] OVEREXPRESSION AND PURIFICATION OF THE RECOMBINANT CA-2+-BINDING PROTEIN, APOAEQUORIN
    INOUYE, S
    AOYAMA, S
    MIYATA, T
    TSUJI, FI
    SAKAKI, Y
    [J]. JOURNAL OF BIOCHEMISTRY, 1989, 105 (03) : 473 - 477