SIMPLE HPLC METHOD FOR THE DETERMINATION OF THYMOQUINONE IN BLACK SEED OIL (NIGELLA-SATIVA LINN)

被引：27

作者：

ABOULENEIN, HY

ABOUBASHA, LI

机构：

[1] Bioanalytical Drug Development Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Centre, Riyadh 112111

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 05期

关键词：

D O I：

10.1080/10826079508010400

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and reliable isocratic normal phase HPLC method for the determination of thymoquinone in black seed oil (Nigella Sativa Linn, Ranculaceae) is described. After oil extraction with methanol, thymoquinone is analyzed using Econosphere CN column. The mobile phase consists of hexane:2 propanol (99:1 v/v), thymoquinone is monitored by UV detection at 295 nm. This method is quite specific and sufficiently sensitive with a lower limit of 5 nmoles/ml, within day and between-day assays showed variation coefficient below 5%.

引用

页码：895 / 902

页数：8

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[10] THE RESPIRATORY EFFECTS OF THE VOLATILE OIL OF THE BLACK SEED (NIGELLA-SATIVA) IN GUINEA-PIGS - ELUCIDATION OF THE MECHANISM(S) OF ACTION [J].

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