ANALYSIS OF THE GENE-CLUSTER ENCODING CAROTENOID BIOSYNTHESIS IN ERWINIA-HERBICOLA EHO13

被引：33

作者：

TO, KY ^{[1
]}

LAI, EM ^{[1
]}

LEE, LY ^{[1
]}

LIN, TP ^{[1
]}

HUNG, CH ^{[1
]}

CHEN, CL ^{[1
]}

CHANG, YS ^{[1
]}

LIU, ST ^{[1
]}

机构：

[1] CHANG GUNG MED COLL,DEPT MICROBIOL & IMMUNOL,MOLEC GENET LAB,TAYUAN 333,TAIWAN

来源：

MICROBIOLOGY-SGM | 1994年 / 140卷

关键词：

ERWINIA HERBICOLA; CAROTENOID BIOSYNTHESIS; OPERON; GENE CLUSTER;

D O I：

10.1099/13500872-140-2-331

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Erwinia herbicola is known to synthesize carotenoids and gives an orange-coloured phenotype. These carotenoids play a role in the protection of the cells from the damage caused by near-UV irradiation in nature. The genes encoding these carotenoids in E. herbicola Eho13 are clustered in a 7 kb DNA fragment. The complete sequence of this fragment has been determined. DNA sequence analysis revealed that the entire sequence contains at least five genes, which are transcribed in the same direction. These five genes are organized in the order crtE-crtX-crtY-crtI-crtB. A gene fusion study showed that two different regions in this 7 kb gene cluster contain promoter activity. Primer-extension analysis identified two transcription start sites, located 147 bp upstream from the first gene of the cluster, crtE, and within the last gene of the cluster, crtB. An RNA-PCR study suggested that the five crt genes were organized in an operon and were transcribed from the promoter upstream from crtE.

引用

页码：331 / 339

页数：9

共 31 条

[1]

AIBA H, 1981, J BIOL CHEM, V256

[2] CONSERVED ENZYMES MEDIATE THE EARLY REACTIONS OF CAROTENOID BIOSYNTHESIS IN NONPHOTOSYNTHETIC AND PHOTOSYNTHETIC PROKARYOTES [J].

ARMSTRONG, GA ;

ALBERTI, M ;

HEARST, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (24) :9975-9979

[3]

BARTLEY GE, 1992, J BIOL CHEM, V267, P5036

[4] MOLECULAR-CLONING AND EXPRESSION IN PHOTOSYNTHETIC BACTERIA OF A SOYBEAN CDNA CODING FOR PHYTOENE DESATURASE, AN ENZYME OF THE CAROTENOID BIOSYNTHESIS PATHWAY [J].

BARTLEY, GE ;

VIITANEN, PV ;

PECKER, I ;

CHAMOVITZ, D ;

HIRSCHBERG, J ;

SCOLNIK, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) :6532-6536

[5]

Beer SV, 1984, ACTA HORTIC HAGUE, V151, P195, DOI DOI 10.17660/ACTAH0RTIC.1984.151.24

[6] CHARACTERISTICS OF ERWINIA-LIKE ORGANISMS FOUND IN PLANT MATERIAL [J].

BILLING, E ;

BAKER, LAE .

JOURNAL OF APPLIED BACTERIOLOGY, 1963, 26 (01) :58-&

[7] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[8]

CARATTOLI A, 1991, J BIOL CHEM, V266, P5854

[9] PLASMID-BORNE DETERMINANTS OF PIGMENTATION AND THIAMINE PROTOTROPHY IN ERWINIA-HERBICOLA [J].

GANTOTTI, BV ;

BEER, SV .

JOURNAL OF BACTERIOLOGY, 1982, 151 (03) :1627-1629

[10]

GOODFELLOW M, 1976, J GEN MICROBIOL, V97, P219, DOI 10.1099/00221287-97-2-219

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