SOME PROPERTIES OF TRANSGLYCOSYLATION ACTIVITY OF SESAME BETA-GLUCOSIDASE

被引：16

作者：

KURIYAMA, K

TSUCHIYA, K

MURUI, T

机构：

[1] Research Laboratory, The Nisshin Oil Mills, Ltd., Yokohama-shi, 221, 1-3, Chiwaka-cho, Kanagawa-ku

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 06期

关键词：

D O I：

10.1271/bbb.59.1142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transglycosylation reaction of partially purified beta-glucosidase from sesame seeds with cellobiose is described. Sesame beta-glucosidase was partially purified by ammonium sulfate fractionation and gel filtration. The molecular weight of the enzyme was 200,000 by gel filtration. Sesame beta-glucosidase showed strong transfer activity to synthesize the trisaccharide from cellobiose. The optimum pH and temperature of the transglycosylation reaction were pH 4.0 and 60 degrees C.

引用

页码：1142 / 1143

页数：2