HETEROLOGOUS EXPRESSION AS A TOOL FOR GENE IDENTIFICATION AND ANALYSIS

被引：15

作者：

GROSS, G ^{[1
]}

HAUSER, H ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH, DEPT GENE REGULAT & DIFFERENTIAT, D-38124 BRAUNSCHWEIG, GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1995年 / 41卷 / 2-3期

关键词：

GENE EXPRESSION; MAMMALIAN CELL; PROKARYOTE; TRANSLATION; INITIATION; TRANSCRIPTION; RNA STABILITY; VECTOR CONSTRUCTION;

D O I：

10.1016/0168-1656(95)00070-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

These days, genome research mainly concerns the accumulation of sequence data and their theoretical interpretation based on analogies to known genes, proteins and structures. However, a final identification of gene function can only be verified by experimental data. One step in this process is the expression of the isolated gene in pro- and eukaryotes. In this article we will review some of the basic features of expression in Escherichia coli and mammalian cells that are relevant to the design of expression experiments. Emphasis is put on the first instance of attaining a high enough level of expression in order to be able to detect the cellular effects or to isolate the product of the transferred gene.

引用

页码：91 / 110

页数：20

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