SUB-MICROLITER FLOW-THROUGH CUVETTE FOR FLUORESCENCE MONITORING OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC EFFLUENTS

被引：106

作者：

HERSHBERGER, LW ^{[1
]}

CALLIS, JB ^{[1
]}

CHRISTIAN, GD ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT CHEM,SEATTLE,WA 98195

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 09期

关键词：

D O I：

10.1021/ac50045a021

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A new sub-microliter flow-through cuvette suitable for monitoring liquid chromatographic effluents has been developed and tested. The cuvette is based on the sheath flow principle, in which the chromatographic effluent is injected into the center of an ensheathing solvent stream but does not mix with it because laminar flow conditions are maintained. The optical volume of the cuvette is easily varied by adjusting the relative flow rates of sheath and sample. The cuvette has been tested for stability, reproducibility, and dynamic range using porphyrins as test compounds. The detection limit for mesoporphyrin IX dimethyl ester was 8 X 10-10 M when the cuvette was operated with an effective detection volume of 53 nL. © 1979, American Chemical Society. All rights reserved.

引用

页码：1444 / 1446

页数：3

共 11 条

[1]

A DEVICE FOR COUNTING SMALL PARTICLES SUSPENDED IN A FLUID THROUGH A TUBE [J].