ISOTACHOPHORETIC ZONE FORMATION OF SERUM-ALBUMIN IN DIFFERENT FREE FLUID ELECTROPHORESIS INSTRUMENTS

被引:21
作者
THORMANN, W [1 ]
FIRESTONE, MA [1 ]
SLOAN, JE [1 ]
LONG, TD [1 ]
MOSHER, RA [1 ]
机构
[1] UNIV ARIZONA,CTR SEPARAT SCI,TUCSON,AZ 85721
关键词
D O I
10.1002/elps.1150110405
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The isotachophoretic behavior of a model protein, serum albumin, was examined (i) by computer simulation, (ii) by capillary isotachophoresis in HPE 100 and Tachophor 2127, (iii) by continuous flow isotachophoresis in Elphor VaP 22 and the BIO‐STREAM Separator and (iv) by recycling isotachophoresis in an apparatus of our own design. Variations in monitored zone shapes can be explained by differences in engineering aspects and fluid stabilization principles of the instruments. Copyright © 1990 VCH Verlagsgesellschaft mbH
引用
收藏
页码:298 / 304
页数:7
相关论文
共 17 条