PHOSPHORYLATION OF THE ETS-2 PROTEIN - REGULATION BY THE T-CELL ANTIGEN RECEPTOR-CD3 COMPLEX

被引：48

作者：

FUJIWARA, S ^{[1
]}

KOIZUMI, S ^{[1
]}

FISHER, RJ ^{[1
]}

BHAT, NK ^{[1
]}

PAPAS, TS ^{[1
]}

机构：

[1] PROGRAM RESOURCES INC, NUCL ACID PROT SYNTH LAB, FREDERICK, MD 21701 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 03期

关键词：

D O I：

10.1128/MCB.10.3.1249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphorylation of the human ets-2 protein in response to mitogenic signals to T lymphocytes was investigated in Jurkat cells. Activation of the cells by antibodies against the T-cell antigen receptor-CD3 complex or by concanavalin A was followed within 5 min by increased phosphorylation of the protein, as shown by a mobility shift of the protein from 54 to 56 kilodaltons in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased incorporation of 32P. The Ca2+ ionophores A23187 and ionomycin were able to mimic this effect, suggesting that this phosphorylation is mediated by Ca2+.

引用

页码：1249 / 1253

页数：5

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