ISOLATION OF BOVINE ZONAE PELLUCIDAE FROM OVARIES WITH COLLAGENASE - ANTIGENIC AND SPERM RECEPTOR PROPERTIES

被引：10

作者：

GWATKIN, RBL

WILLIAMS, DT

MEYENHOFER, M

机构：

[1] Merck Institute for Therapeutic Research, Rahway, New Jersey

来源：

GAMETE RESEARCH | 1979年 / 2卷 / 02期

关键词：

collagenase; receptor for sperm; zona isolation;

D O I：

10.1002/mrd.1120020210

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Zonae pellucidae were collected from bovine ovaries by chopping, dispersing the chopped tissue with collagenase, sieving through nylon mesh screens, and pipetting. The zonae were free of corona cell processes when examined under the scanning electron microscope. Solutions of zonae obtained with collagenase exhibited the same antigenic and sperm receptor properties as those obtained without enzyme treatment. Copyright © 1979 Alan R. Liss, Inc.

引用

页码：187 / 192

页数：6

共 11 条

[1] TECHNIQUES FOR THE PRESERVATION OF 3-DIMENSIONAL STRUCTURE IN PREPARING SPECIMENS FOR THE ELECTRON MICROSCOPE [J].

ANDERSON, TF .

TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES, 1951, 13 (04) :130-134

[2] PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES [J].

DULBECCO, R ;

VOGT, M .

JOURNAL OF EXPERIMENTAL MEDICINE, 1954, 99 (02) :167-182

[3]

DUNBAR BS, 1978, J CELL BIOL, V79, pA163

[4] IMMUNIZATION OF FEMALE RABBITS WITH HEAT-SOLUBILIZED BOVINE ZONAE - PRODUCTION OF ANTI-ZONA ANTIBODY AND INHIBITION OF FERTILITY [J].

GWATKIN, RBL ;

WILLIAMS, DT .

GAMETE RESEARCH, 1978, 1 (01) :19-26

[5] BOVINE AND HAMSTER ZONA SOLUTIONS EXHIBIT RECEPTOR ACTIVITY FOR CAPACITATED BUT NOT FOR NON-CAPACITATED SPERM [J].

GWATKIN, RBL ;

WILLIAMS, DT .

GAMETE RESEARCH, 1978, 1 (3-4) :259-263

[6]

GWATKIN RBL, 1978, MOL BASIS CELL CELL, V14, P363

[7]

GWATKIN RBL, 1978, RECENT ADV REPRODUCT

[8] ADHESION OF CELLS TO SURFACES COATED WITH POLYLYSINE - APPLICATIONS TO ELECTRON-MICROSCOPY [J].

MAZIA, D ;

SCHATTEN, G ;

SALE, W .

JOURNAL OF CELL BIOLOGY, 1975, 66 (01) :198-200

[9] ELECTRON MICROSCOPIC STUDIES ON OVARIAN OOCYTES AND UNFERTILIZED TUBAL OVA IN THE RAT [J].

ODOR, DL .

JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY, 1960, 7 (03) :567-&

[10] SIMPLE METHOD FOR THE ISOLATION OF A LARGE NUMBER OF OVA FROM PIG OVARIES [J].

OIKAWA, T .

GAMETE RESEARCH, 1978, 1 (3-4) :265-267

← 1 2 →