NADPH - A STIMULATORY COFACTOR FOR NITRIC OXIDE-INDUCED ADP-RIBOSYLATION REACTION

被引：14

作者：

BRUNE, B

DIMMELER, S

LAPETINA, EG

机构：

[1] BURROUGHS WELLCOME CO,DIV CELL BIOL,3030 CORNWALLIS RD,RES TRIANGLE PK,NC 27709

[2] UNIV CONSTANCE,FAC BIOL,DEPT BIOL CHEM,W-7750 CONSTANCE,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91854-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An endogenous ADP-ribosyltransferase is present in the cytosolic fraction of human platelets. Agents known to release nitric oxide activated this ADP-ribosylation reaction in a cGMP-independent fashion. This enzymatic activity was further enhanced by the addition of NADPH to the platelet cytosolic fraction. Interestingly, NADPH was anable to replace DTT, which has been described as an essential cofactor. Our results indicate that NADPH is a stimulatory factor of the endogenous ADP-ribosylation reaction. NADPH shifts the dose-response curve of NO to the left and possibly increases, in this way, the ADP-ribosylation reaction under physiological conditions. © 1992.

引用

页码：1166 / 1171

页数：6

共 16 条

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Baenziger N L, 1974, Methods Enzymol, V31, P149

[2] ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME [J].