NONRADIOACTIVE PCR ENZYME-LINKED-IMMUNOSORBENT-ASSAY METHOD FOR DETECTION OF HUMAN CYTOMEGALOVIRUS DNA

被引：25

作者：

ALLEN, RD ^{[1
]}

PELLETT, PE ^{[1
]}

STEWART, JA ^{[1
]}

KOOPMANS, M ^{[1
]}

机构：

[1] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIAL DIS,ATLANTA,GA 30333

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 03期

关键词：

D O I：

10.1128/JCM.33.3.725-728.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.

引用

页码：725 / 728

页数：4

共 13 条

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