BETA-CYTOPLASMIC AND GAMMA-CYTOPLASMIC ACTINS ARE DIFFERENTIALLY THERMOSTABILIZED BY MGADP - GAMMA-ACTIN BINDS MGADP MORE STRONGLY

Using the technique of thermal denaturation in a temperature gradient followed by two-dimensional gel electrophoresis of the fractions, the thermostabilities of the cytoplasmic actins have been investigated. Addition of 5 mM MgADP (but not NADH or CaCl2) to stripped, radiolabeled leukocyte lysate thermostabilizies the actins by about 16°C relative to stripped lysate alone, producing a slightly stronger stabilization in γ actin. This result will allow preparative fractionation of β and γ actins and additional biochemical characterization of their differences. © 1979.