HOMOLOGOUS RECOMBINATION IN A MAMMALIAN PLASMID

被引:13
作者
KITAMURA, Y
YOSHIKURA, H
KOBAYASHI, I
机构
[1] UNIV TOKYO,INST MED SCI,DEPT MOLEC BIOL,MINATO KU,TOKYO 108,JAPAN
[2] UNIV TOKYO,FAC MED,DEPT BACTERIOL,TOKYO 113,JAPAN
来源
MOLECULAR & GENERAL GENETICS | 1990年 / 222卷 / 2-3期
关键词
Bovine papillomavirus type 1; Non-reciprocality; Plasmid rescue; Single clone analysis;
D O I
10.1007/BF00633816
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bovine papillomavirus (BPV) shuttle vectors replicate as a circular plasmid in mouse cell nuclei without impairing host cell viability. We used these vectors to analyze homologous recombination in mammalian cells. When several BPV-based plasmids carrying direct repeats were introduced into C127 cells, we detected many recombinant plasmid molecules that have lost the sequence between the repeats. Many recombinant type molecules as well as parental type molecules were detected in all the cell clones isolated for analysis. Sequencing after rescue of the plasmid in Escherichia coli showed that most of the recombinants were from accurate homologous recombination. When the repeats on the plasmid were in inverted orientation, no crossing-over type products were detected. We discuss possible mechanisms that explain these features. © 1990 Springer-Verlag.
引用
收藏
页码:185 / 191
页数:7
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