PKA VALUES AND PARTITION-COEFFICIENTS OF NITROXIDE SPIN PROBES FOR MEMBRANE BIOENERGETICS MEASUREMENTS

被引：24

作者：

FUCHS, J

NITSCHMANN, WH

PACKER, L

HANKOVSZKY, OH

HIDEG, K

机构：

[1] UNIV CALIF BERKELEY LAWRENCE BERKELEY LAB, MEMBRANE BIOENERGET GRP, BERKELEY, CA 94720 USA

[2] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA

[3] UNIV PECS, CENT LAB CHEM, H-7643 PECS, HUNGARY

[4] JW GOETHE UNIV KLINIKUM, ZENTRUM DERMATOL & VENEROL, ABT 2, W-6000 FRANKFURT 70, GERMANY

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 10卷 / 06期

基金：

美国国家航空航天局;

关键词：

Dissociation constants; Mitochondria; Nitroxides; Partition coefficients; PH indicators; Synechococcus;

D O I：

10.3109/10715769009149900

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Knowledge of pKa's is necessary to calculate intracellular/intravesicular pH values from nitroxide accumulation in cells or vesicles as detected with electron spin resonance (ESR) spectroscopy. pKa values were confirmed in lipid vesicles of known internal pH. To help select probes that do not accumulate in lipid membranes, octanol/buffer partition coefficients of uncharged nitroxides were determined. As an application of selected probes, pH gradients and internal aqueous volumes were analyzed in mitochondria (one internal compartment) and in the cyanobacterium Synechococcus 6311 (two internal compartments). The combination of 3-carboxy-, 3-amino- and 3-aminocarbonyl-2,2,5,5-tetramethylpyrrolidin-1-yloxyl was found to be most satisfactory for determinations of internal pH and volumes. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：315 / 323

页数：9

共 27 条

[1] REDUCTION AND DESTRUCTION RATES OF NITROXIDE SPIN PROBES [J].