APPLICATION OF C-13(OMEGA-1)-HALF-FILTERED [H-1,H-1]-NOESY FOR STUDIES OF A COMPLEX FORMED BETWEEN DNA AND A C-13-LABELED MINOR-GROOVE-BINDING DRUG

被引：9

作者：

LEUPIN, W ^{[1
]}

OTTING, G ^{[1
]}

AMACKER, H ^{[1
]}

WUTHRICH, K ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,INST MOLEK BIOL & BIOPHYS,CH-8093 ZURICH,SWITZERLAND

来源：

FEBS LETTERS | 1990年 / 263卷 / 02期

关键词：

Drug/oligodeoxyribonucleotide complex; Intermolecular nuclear Overhauser efrect; Nuclear magnetic resonance; [!sup]1[!/sup]H; !sup]1[!/sup]H]-NOESY with [!sup]13[!/sup]C(ω[!sub]1[!/sub])-half-filter;

D O I：

10.1016/0014-5793(90)81402-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complex formed between the anticancer drug 4-[p-[p-(4-quinolylamino)benzamido]anilino]pyridine (SN 6999) and the decadeoxyribonucleoside nonaphosphate d-(GCATTAATGC)2 was investigated using two-dimensional nuclear Overhauser enhancement spectroscopy (NOESY) with a 13C(ω1)-half-filter. The two quaternary methyl groups in SN 6999 had been labeled with 13C for these experiments. The simplified subspectra of [1H,1H]-NOESY obtained with this procedure greatly facilitate the identification and assignment of intermolecular NOEs. Quite generally, the combined use of isotope labeling and heteronuclear filters in [1H,1H]-NOESY provides an improved experimental basis for structural studies of drug/ DNA complexes. © 1990.

引用

页码：313 / 316

页数：4

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