DISCRIMINATION OF CORYNEBACTERIUM-GLUTAMICUM, BREVIBACTERIUM-FLAVUM AND BREVIBACTERIUM-LACTOFERMENTUM BY RESTRICTION PATTERN-ANALYSIS OF DNA ADJACENT TO THE HOM GENE

被引：13

作者：

EIKMANNS, BJ ^{[1
]}

KIRCHER, M ^{[1
]}

REINSCHEID, DJ ^{[1
]}

机构：

[1] DEGUSSA AG, HALLE KUNSEBECK, GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 82卷 / 02期

关键词：

CORYNEBACTERIUM; BREVIBACTERIUM; HOM GENE; TREHALOSE; ETHIONINE;

D O I：

10.1016/0378-1097(91)90333-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Different strains of Corynebacterium glutamicum, Brevibacterium flavum, and Brevibacterium lactofermentum were analysed for restriction fragment length polymorphism using the homoserine dehydrogenase gene (hom) as a probe. The hybridization patterns obtained PvuII- or Asp 700-restriction of chromosomal DNA were specific and distinguishable for each of the three species and identical for the different strains of each species. Thus, the method employed allows rapid distinction of Corynebacterium glutamicum, Brevibacterium flavum, and Brevibacterium lactofermentum. The former species could also be discriminated from the latter two by its resistance to 0.5 g/l of the methionine analog ethionine.

引用

页码：203 / 207

页数：5

共 20 条

[1] TAXONOMICAL STUDIES ON GLUTAMIC ACID-PRODUCING BACTERIA [J].