IN-SITU HYBRIDIZATION UTILIZING A Y-CHROMOSOME DNA-PROBE - USE AS A CELL MARKER FOR HEPATOCELLULAR TRANSPLANTATION

被引:9
作者
ECKERT, JW
BUERKLE, CJ
MAJOR, AM
FINEGOLD, MJ
BRANDT, ML
机构
[1] TEXAS CHILDRENS HOSP,HOUSTON,TX 77030
[2] BAYLOR COLL MED,DEPT SURG,HOUSTON,TX 77030
[3] BAYLOR COLL MED,DEPT PATHOL,HOUSTON,TX 77030
关键词
D O I
10.1097/00007890-199501150-00019
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Research in hepatocellular gene therapy requires a consistently reproducible cell marker to detect transplanted hepatocytes. We have used a Y-specific genomic DNA probe to accomplish this goal. This technique enables the identification of transplanted male cells in recipient female tissues. Donor hepatocytes from male mice were transplanted into female mice via splenic injection. Recipient mouse livers were harvested 1, 24, and 48 hr after transplant. Transplanted (male) hepatocytes were detected in liver biopsy sections using in situ hybridization with the Y chromosome probe.
引用
收藏
页码:109 / 111
页数:3
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