PURIFICATION AND SOME PROPERTIES OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, PSEUDOMONAS-HYDROGENOTHERMOPHILA STRAIN-TH-1

被引：5

作者：

CHUNG, SY

YOKOYAMA, K

GOMI, M

TEAUMROONG, N

ISHII, M

IGARASHI, Y

KODAMA, T

机构：

[1] UNIV TOKYO, GRAD SCH AGR & LIFE SCI, DEPT BIOTECHNOL, BUNKYO KU, TOKYO 113, JAPAN

[2] INST PHYS & CHEM RES, MICROBIOL LAB, WAKO, SAITAMA 35101, JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 78卷 / 06期

关键词：

D O I：

10.1016/0922-338X(94)90049-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ribulose 1,5-bisphosphate carboxylase/oxygenase was purified from a thermophilic hydrogen-oxidizing bacterium, Pseudomonas hydrogenothermophila strain TH-1. The enzyme was an L(8)S(8)-type hexadecamer with a molecular mass of 560 kDa. The specific activity of the purified enzyme was about 6.5 mu mol/mg and stable even by heating at 60 degrees C for 30 min. The K-m values for RuBP, CO2, and Mg++ were 1.03 mu M, 0.086 mM and 0.92 mM, respectively.

引用

页码：469 / 471

页数：3

共 9 条

[1]

Andrews TJ, 1987, BIOCH PLANTS, V10, P131

[2]

GLOVER HE, 1989, INT REV CYTOL, V115, P67

[3] STUDIES ON HYDROGEN-UTILIZING MICROORGANISMS .6. GROWTH AND TAXONOMY OF THERMOPHILIC HYDROGEN BACTERIA [J].