GENETIC-VARIATION IN STAPHYLOCOCCUS-AUREUS COAGULASE GENES - POTENTIAL AND LIMITS FOR USE AS EPIDEMIOLOGIC MARKER

被引:75
作者
SCHWARZKOPF, A
KARCH, H
机构
关键词
D O I
10.1128/JCM.32.10.2407-2412.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To perform coagulase gene typing, the repeated units encoding hypervariable regions of the Staphylococcus aureus coagulase gene were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In order to assess the discriminatory power of this typing method, 30 epidemiologically unrelated S. aureus strains which differed by their pulsed-field gel electrophoresis patterns were examined. Although 18 of the 30 strains had unique and unshared AluI RFLP patterns, there were only four observed patterns in the remaining 12 strains. This finding indicated that unrelated strains may share identical AluI RFLP patterns. To elucidate the degree of genetic variation in the C-terminus-encoding loci within the coagulase genes, the PCR products of these 12 strains were subjected to Tag polymerase-mediated sequencing. Sequence analysis confirmed the AluI recognition sites in each of the four RFLP groups and demonstrated that AluI appears to yield the highest RFLP in restriction enzyme analysis. By their DNA sequences the majority of strains sharing common AluI groups could be clearly differentiated from each other and revealed between 93.2 and 98.5% homology. When we determined the nucleotide sequences of two strains after six subcultivations no significant alterations were observed. Because the discriminatory power of the current coagulase gene typing method is not great enough to be used as the sole method to type S. aureus, additional techniques are necessary. Sequence analysis of the repeated unit-encoding region for the typing of S. aureus may be potentially useful as an alternative to other current molecular typing techniques.
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页码:2407 / 2412
页数:6
相关论文
共 20 条
[1]   EPIDEMIOLOGIC TYPING OF STAPHYLOCOCCUS-AUREUS BY DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS OF RIBOSOMAL-RNA GENES - ELUCIDATION OF THE CLONAL NATURE OF A GROUP OF BACTERIOPHAGE-NONTYPABLE, CIPROFLOXACIN-RESISTANT, METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS ISOLATES [J].
BLUMBERG, HM ;
RIMLAND, D ;
KIEHLBAUCH, JA ;
TERRY, PM ;
WACHSMUTH, IK .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (02) :362-369
[2]   EPIDEMIOLOGIC MARKERS FOR EPIDEMIC STRAIN AND CARRIER ISOLATES IN AN OUTBREAK OF NOSOCOMIAL OXACILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS [J].
BOUVET, A ;
FOURNIER, JM ;
AUDURIER, A ;
BRANGER, C ;
ORSONI, A ;
GIRARD, C .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (06) :1338-1341
[3]   METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS [J].
BRUMFITT, W ;
HAMILTONMILLER, J .
NEW ENGLAND JOURNAL OF MEDICINE, 1989, 320 (18) :1188-1196
[4]   DNA POLYMORPHISMS IN METHICILLIN-SUSCEPTIBLE AND METHICILLIN-RESISTANT STRAINS OF STAPHYLOCOCCUS-AUREUS [J].
CARLESNURIT, MJ ;
CHRISTOPHLE, B ;
BROCHE, S ;
GOUBY, A ;
BOUZIGES, N ;
RAMUZ, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) :2092-2096
[5]   RAPID FIELD INVERSION GEL-ELECTROPHORESIS IN COMBINATION WITH AN RIBOSOMAL-RNA GENE PROBE IN THE EPIDEMIOLOGIC EVALUATION OF STAPHYLOCOCCI [J].
GOERING, RV ;
DUENSING, TD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :426-429
[6]   MOLECULAR TYPING OF STAPHYLOCOCCUS-AUREUS ON THE BASIS OF COAGULASE GENE POLYMORPHISMS [J].
GOH, SH ;
BYRNE, SK ;
ZHANG, JL ;
CHOW, AW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1642-1645
[7]   USE OF A RIBOSOMAL-RNA GENE PROBE FOR THE EPIDEMIOLOGIC-STUDY OF METHICILLIN AND CIPROFLOXACIN RESISTANT STAPHYLOCOCCUS-AUREUS [J].
HADORN, K ;
LENZ, W ;
KAYSER, FH ;
SHALIT, I ;
KRASEMANN, C .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1990, 9 (09) :649-653
[8]   GENOMIC DNA FINGERPRINTING BY PULSED-FIELD GEL-ELECTROPHORESIS AS AN EPIDEMIOLOGIC MARKER FOR STUDY OF NOSOCOMIAL INFECTIONS CAUSED BY METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS [J].
ICHIYAMA, S ;
OHTA, M ;
SHIMOKATA, K ;
KATO, N ;
TAKEUCHI, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (12) :2690-2695
[9]   NUCLEOTIDE AND DEDUCED AMINO-ACID SEQUENCES OF STAPHYLOCOAGULASE GENE FROM STAPHYLOCOCCUS-AUREUS STRAIN 213 [J].
KAIDA, S ;
MIYATA, T ;
YOSHIZAWA, Y ;
IGARASHI, H ;
IWANAGA, S .
NUCLEIC ACIDS RESEARCH, 1989, 17 (21) :8871-8871
[10]   NUCLEOTIDE-SEQUENCE OF THE STAPHYLOCOAGULASE GENE - ITS UNIQUE COOH-TERMINAL-8 TANDEM REPEATS [J].
KAIDA, S ;
MIYATA, T ;
YOSHIZAWA, Y ;
KAWABATA, S ;
MORITA, T ;
IGARASHI, H ;
IWANAGA, S .
JOURNAL OF BIOCHEMISTRY, 1987, 102 (05) :1177-1186