INHIBITION OF ENZYME-ANTIENZYME INTERACTION BY TRYPTIC DIGESTS OF ENZYME

被引:6
作者
MINATOGAWA, S
RUSSELL, PJ
机构
[1] Department of Biochemistry, University of Kansas Medical Center, Kansas City
关键词
D O I
10.1016/0006-291X(68)90029-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper presents evidence that peptides of tryptic digests of adenylate kinases (EC 2.7.4.3) from yeast (Chiu et al., 1967) and rabbit muscle (Noda and Kuby, 1957) are capable of inhibiting the rate of interaction of these enzymes with their respective antisera (Minatogawa et al., 1967). The inhibitions appeared specific and affected only the rate of interaction and not the final quantity which interacted. The findings suggest that a kinetic approach to the search for active peptide fragments is a sensitive method which permits the detection of antigen fragments which reversibly combine with antibody. © 1968.
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页码:48 / +
页数:1
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