GUINEA PIG ASPARAGINE SYNTHETASE

被引：24

作者：

HOLCENBERG, JS

机构：

[1] Division of Clinical Pharmacology, Departments of Medicine and Pharmacology, University of' Washington School of Medicine, Seattle, WA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 185卷 / 01期

关键词：

D O I：

10.1016/0005-2744(69)90297-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The partial purification and properties of guinea pig asparagine synthetase (l-aspartate: ammonia ligase (AMP), EC 6.3.1.1) are described. The enzyme activity is present largely in the high-speed supernatant fractions and is found in extracts from all tissues with the highest specific activities in extracts from spleen, liver, intestine and lymph nodes. High concentrations of (NH4)2SO4 or K2SO4 with glutamine are required to demonstrate this activity in crude enzyme preparations. l-Asparagine appreciably inhibits the reaction in the presence of glutamine but not in the presence of high concentrations of (NH4)2SO4. © 1969.

引用

页码：228 / +

页数：1

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