ANATOMICAL LOCALIZATION OF CALMODULIN MESSENGER-RNA IN THE RAT-BRAIN WITH CLONED CDNA AND SYNTHETIC OLIGONUCLEOTIDE PROBES

被引：52

作者：

ROBERTSLEWIS, JM

CIMINO, M

KRAUSE, RG

TYRRELL, DF

DAVIS, LG

WEISS, B

LEWIS, ME

机构：

[1] MED COLL PENN,EASTERN PENN PSYCHIAT INST,DEPT PHARMACOL,DIV NEUROPSYCHOPHARMACOL,PHILADELPHIA,PA 19129

[2] DUPONT CO,EXPTL STN,DEPT MED PROD,WILMINGTON,DE 19898

来源：

SYNAPSE | 1990年 / 5卷 / 03期

关键词：

Calcium‐binding protein; In situ hybridization histochemistry;

D O I：

10.1002/syn.890050311

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Calmodulin is a small, acidic calcium‐binding protein that regulates a number of calcium‐dependent enzyme activities and is thought to be involved in neurotransmission to begin to explore further the regulation of this important protein in the brain, we have cloned a rat calmodulin cDNA and designed an oligonucleotide probe based on this sequence. Both the cDNA and oligonucleotide probes revealed a markedly heterogeneous distribution of hybridization signal for calmodulin mRNA in the rat brain. The greatest apparent abundance of mRNA for calmodulin was seen in the hippocampus and cerebral cortex, whereas many brain regions showed relatively low hybridization signal, including the striatum and portions of the hypothalamus and brainstem. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：247 / 254

页数：8

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