THE DETERMINATION OF ASIALOGLYCOFORMS OF SERUM GLYCOPROTEINS BY LECTIN BLOTTING WITH RICINUS-COMMUNIS AGGLUTININ

被引：8

作者：

LUNDY, FT ^{[1
]}

WISDOM, GB ^{[1
]}

机构：

[1] QUEENS UNIV BELFAST,CTR MED BIOL,DIV BIOCHEM,BELFAST BT9 7BL,ANTRIM,NORTH IRELAND

来源：

CLINICA CHIMICA ACTA | 1992年 / 205卷 / 03期

关键词：

ASIALOGLYCOPROTEINS; SERUM PROTEINS; LECTIN BLOTTING; RCA120; LECTIN;

D O I：

10.1016/0009-8981(92)90059-Y

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptide were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (alpha-2-macroglobulin, transferrin. alpha-1-antitrypsin, alpha-1-antichymotrypsin and haptoglobin beta-chain). The asialoform of the alpha-1-trypsin Inhibitor was found to be decreased in inflammation.

引用

页码：187 / 195

页数：9

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