BINDING OF CYTOSOLIC PROTEIN FROM CULTURED RAT HEPATOCYTES TO THE 3'-END OF PHOSPHOENOLPYRUVATE CARBOXYKINASE MESSENGER-RNA - SIGNIFICANCE FOR PROTEIN-MEDIATED MESSENGER-RNA STABILIZATION

被引：10

作者：

CHRIST, B

HEISE, T

JUNGERMANN, K

机构：

[1] Institut für Biochemie, Universität Göttingen, D-3400 Göttingen

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90679-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro transcripts of the 3′-non-translated region of the phosphoenolpyruvate carboxykinase (PCK) gene both in "sense" and "antisense" orientation bound cytosolic protein from cultured rat hepatocytes as demonstrated by electrophoretic mobility shift assay. Binding of cytosolic protein was increased 3-fold and PCK mRNA was enhanced 10-fold by treatment of the hepatocytes with 10 nM glucagon. The similar time course of the glucagon-induced increase in protein binding to PCK mRNA 3′-end and in PCK mRNA suggests that protein binding might be involved in the stabilization of PCK mRNA. © 1991.

引用

收藏

页码：1273 / 1282

页数：10

相关论文

共 25 条

[1] HUMAN VASCULAR ENDOTHELIAL-CELLS, GRANULOPOIESIS, AND THE INFLAMMATORY RESPONSE [J].

BAGBY, GC ;

SHAW, G ;

SEGAL, GM .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1989, 93 (02) :S48-S52

[2] NUCLEASE ACTIVITY ASSOCIATED WITH MAMMALIAN MESSENGER-RNA IN ITS NATIVE-STATE - POSSIBLE BASIS FOR SELECTIVITY IN MESSENGER-RNA DECAY [J].

BANDYOPADHYAY, R ;

COUTTS, M ;

KROWCZYNSKA, A ;

BRAWERMAN, G .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (05) :2060-2069

[3]

BEALE EG, 1985, J BIOL CHEM, V260, P748

[4] THE POLY(A)-POLY(A)-BINDING PROTEIN COMPLEX IS A MAJOR DETERMINANT OF MESSENGER-RNA STABILITY INVITRO [J].

BERNSTEIN, P ;

PELTZ, SW ;

ROSS, J .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (02) :659-670

[5] POLY(A), POLY(A) BINDING-PROTEIN AND THE REGULATION OF MESSENGER-RNA STABILITY [J].

BERNSTEIN, P ;

ROSS, J .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (09) :373-377

[6] DETERMINANTS OF MESSENGER-RNA STABILITY [J].

BRAWERMAN, G .

CELL, 1987, 48 (01) :5-6

[7] IDENTIFICATION OF A COMMON NUCLEOTIDE-SEQUENCE IN THE 3'-UNTRANSLATED REGION OF MESSENGER-RNA MOLECULES SPECIFYING INFLAMMATORY MEDIATORS [J].

CAPUT, D ;

BEUTLER, B ;

HARTOG, K ;

THAYER, R ;

BROWNSHIMER, S ;

CERAMI, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (06) :1670-1674

[8] IRON-RESPONSIVE ELEMENTS - REGULATORY RNA SEQUENCES THAT CONTROL MESSENGER-RNA LEVELS AND TRANSLATION [J].

CASEY, JL ;

HENTZE, MW ;

KOELLER, DM ;

CAUGHMAN, SW ;

ROUAULT, TA ;

KLAUSNER, RD ;

HARFORD, JB .

SCIENCE, 1988, 240 (4854) :924-928

[9] MECHANISM OF THE INHIBITION BY INSULIN OF THE GLUCAGON-DEPENDENT ACTIVATION OF THE PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE IN RAT HEPATOCYTE CULTURES - ACTION ON GENE-TRANSCRIPTION, MESSENGER-RNA LEVEL AND STABILITY AS WELL AS HYSTERESIS EFFECT [J].

CHRIST, B ;

NATH, A ;

JUNGERMANN, K .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1990, 371 (05) :395-402

[10] REGULATION OF THE EXPRESSION OF THE PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE IN CULTURED RAT HEPATOCYTES BY GLUCAGON AND INSULIN [J].

CHRIST, B ;

NATH, A ;

BASTIAN, H ;

JUNGERMANN, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 178 (02) :373-379