MAPPING OF THE PIM-1 ONCOGENE IN MOUSE T-HAPLOTYPES AND ITS USE TO DEFINE THE RELATIVE MAP POSITIONS OF THE TCL LOCI T0(T6) AND TW12 AND THE MARKER TF (TUFTED)

Pim-1 is an oncogene activated in mouse T-cell lymphomas induced by Moloney and AKR mink cell focus (MCF) viruses. Pim-1 was previously mapped to chromosome 17 by somatic cell hybrids, and subsequently to the region between the hemoglobin α-chain pseudogene 4 (Hba-4ps) and the α-crystalline gene (Crya-1) by Southern blot analysis of DNA obtained from panels of recombinant inbred strains. We have now mapped Pim-1 more accurately in t-haplotypes by analysis of recombinant t-chromosomes. The recombinants were derived from Tts6tf t12 parents backcrossed to + tf +tf, and scored for recombination between the loci of T and tf. For simplicity all t-complex lethal genes properly named tck-tx are shortened to tx. The Pim-1 gene was localized 0.6 cM proximal to the tw12 lethal gene, thus placing the Pim-1 gene 5.2 cM distal to the H-2 region in t-haplotypes. Once mapped, the Pim-1 gene was used as a marker for further genetic analysis of t-haplotypes, tw12 is so close to tf that even with a large number of recombinants it was not possible to determine whether it is proximal or distal to tf. Southern blot analysis of DNA from T-tf recombinants with a separation of tw12 and tf indicated that tw12 is proximal to tf. The mapping of two alletic t-lethals, t0 and t6 with respect to tw12 and tf has also been a problem. The map position of t0(t6) was obtained through Southern blot analysis of DNA from a panel of Tts6tf t0 and Tts6tf t6 recombinants with separation of tw12 and t0(t6). t0(t6) was placed distal to tf. Southern blot analysis of the th20 deletion, which covers the locus of tf, indicated that this deletion does not include the Pim-1 gene. © 1991.