FACTORS AFFECTING THE RESULTS OF T-2 MYCOTOXIN ELISA ASSAY

被引：11

作者：

LAAMANEN, I ^{[1
]}

VEIJALAINEN, P ^{[1
]}

机构：

[1] NATL VET INST,DEPT VIROL,SF-00101 HELSINKI,FINLAND

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1992年 / 9卷 / 04期

关键词：

MYCOTOXINS; T-2; ELISA; FALSE REACTIONS;

D O I：

10.1080/02652039209374079

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Certain substances in the sample may increase or decrease the reaction between the enzyme and substrate in ELISA assays. During a survey of T-2 trichothecene in food and animal feed 75% of milled grain samples gave a higher O.D. value in competitive T-2 toxin ELISA than the negative control. In samples spiked with small quantities (10 mug/kg) of T-2 toxin this type of reaction resulted in underestimates of toxin content. However, the effect was weak and, owing to the high sensitivity of the assay, it did not result in false negative reactions. The low efficiency of the carrier solvent and natural peroxidases in food and feed were considered to be the cause of the inaccurate reactions. A few fermented and processed foodstuffs and feed gave positive results in the T-2 toxin ELISA assay, but verification of the results by gas chromatography (GC) showed that the reactions were false. Certain substances in the samples destroyed or decreased the enzyme activity. False positive reactions can be distinguished from correct ones by retesting the extracts in different dilutions.

引用

页码：337 / 343

页数：7

共 9 条

[1]

Abouzied M.M., Azcona J.I., Braselton W.E., Petska J.J., Immunochemical assessment of mycotoxins in 1989 grain foods: Evidence for deoxynivalenol (vomitoxin) contamination, Applied and Environmental Microbiology, 57, pp. 672-677

[2]

Chiba J., Kawamura O., Kajii H., Ohtani K., Nagayama S., Ueno Y., A sensitive enzyme-linked immunosorbent assay for detection of T-2 toxin with monoclonal antibodies, Food Additives and Contaminants, 5, pp. 629-639, (1988)

[3]

Chu F.S., Chen Liang M.Y., Zhang G.S., Production and characterization of antibody against diacetoxyscirpenol, Applied and Environmental Microbiology, 48, pp. 777-780, (1984)

[4]

Fan T.S.L., Xu Y.-C., Chu F.S., Simultaneous analysis of T-2 toxin and HT-2 toxin by an indirect enzyme-linked immunosorbent assay, Journal of the Association of Official Analytical Chemists, 70, pp. 657-661, (1987)

[5]

Karppanen E., Rizzo A., Berg S., Lindfors E., Aho R., Fusarium, Journal of Agricultural Science in Finland, 57, pp. 195-206, (1985)

[6]

Kurstak E., Enzyme Immunodiagnosis, pp. 1-223, (1986)

[7]

Stahr H.M., Hyde W., Martin P.J., Field testing for mycotoxins using the spectrochrom Myco-Quick kit and others, Biodeterioration Research, 2, pp. 295-300, (1989)

[8]

Ueno Y., Trichothecenes as environmental toxicants, Reviews in Environmental Toxicology, pp. 303-341, (1986)

[9]

Ueno Y., Sato N., Ishii K., Sakai H., Tsunoda H., Enomoto M., Biological and chemical detection of trichothecene mycotoxins of Fusarium species, Applied Environmental Microbiology, 25, pp. 699-704, (1973)

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