LOCALIZATION OF NITRIC-OXIDE SYNTHASE WITHIN NONADRENERGIC, NONCHOLINERGIC NERVES IN THE MOUSE ANOCOCCYGEUS

Immunocytochemical staining of whole mount preparations of the mouse anococcygeus muscle, using antibodies to rat brain nitric oxide synthase (NOS), revealed a dense network of NOS-immunoreactive nerve fibres running through the tissue. These fibres were resistant to the sympathetic neurotoxin 6-hydroxydopamine and are therefore likely to be the non-adrenergic nerves which mediate relaxation of this smooth muscle. Further, NOS-immunoreactive fibres were absent following denervation by cold-storage (4-degrees-C; 72 h), which has been shown to abolish non-adrenergic, non-cholinergic (NANC) relaxations. The results provide strong support for the hypothesis that the L-arginine:NO pathway is responsible for the generation of the NANC transmitter in the anococcygeus.