INHIBITION OF INDUCIBLE NITRIC-OXIDE SYNTHASE MESSENGER-RNA EXPRESSION BY BASIC FIBROBLAST GROWTH-FACTOR IN HUMAN MICROGLIAL CELLS

被引：26

作者：

COLASANTI, M

DIPUCCHIO, T

PERSICHINI, T

SOGOS, V

PRESTA, M

LAURO, GM

机构：

[1] UNIV ROME 3,DEPT BIOL,I-00154 ROME,ITALY

[2] SCH MED CAGLIARI,DEPT CYTOMORPHOL,CAGLIARI,ITALY

[3] SCH MED BRESCIA,DEPT BIOMED SCI & BIOTECHNOL,GEN PATHOL & IMMUNOL UNIT,BRESCIA,ITALY

来源：

NEUROSCIENCE LETTERS | 1995年 / 195卷 / 01期

关键词：

HUMAN MICROGLIA; BASIC FIBROBLAST GROWTH FACTOR; INDUCIBLE NO SYNTHASE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; NITRIC OXIDE; TRANSCRIPTIONAL REGULATION;

D O I：

10.1016/0304-3940(95)11778-U

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effect of basic fibroblast growth factor (bFGF) on inducible nitric oxide synthase (iNOS) mRNA expression in human cultured ramified microglial cells was investigated. Using RT-PCR and Southern blot analysis, we found that bFGF prevented the iNOS gene expression as induced by LPS/TNF alpha. Also, bFGF dose-dependently inhibited nitrite levels in treated cell supernatants. That the early presence of bFGF during LPS/TNF alpha induction was essential indicates that iNOS gene expression can be transcriptionally regulated.

引用

页码：45 / 48

页数：4

共 22 条

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