IMMOBILIZATION OF ENZYMES AND MICROBIAL-CELLS USING CARRAGEENAN AS MATRIX

被引：140

作者：

TOSA, T

SATO, T

MORI, T

YAMAMOTO, K

TAKATA, I

NISHIDA, Y

CHIBATA, I

机构：

[1] Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co. Ltd, Osaka, 16-89, Kashima-3-chome, Yodogawa-ku

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 10期

关键词：

D O I：

10.1002/bit.260211002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conditions for the gelation of k ‐carrageenan, which is a new polymer for immobilization of enzymes and microbial cells, were investigated in detail. k ‐Carrageenan was easily induced to gel by contact with metal ions, amines, amino acid derivatives, and water‐miscible organic solvents. By using this property of k ‐carrageenan, the immobilization of enzymes and microbial cells was investigated. Several kinds of enzymes and microbial cells were easily immobilized with high enzyme activities. Immobilized preparations were easily tailor‐made to various shapes such as cube, bead, and membrane. The obtained immobilized preparations were stable, and columns packed with them were used for continuous enzyme reaction for a long period. Their operational stabilities were enhanced by hardening with glutaraldehyde and hexamethylenediamine. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：1697 / 1709

页数：13

共 12 条

[1] [Anonymous], 1977, J SOL-PHASE BIOCHEM, DOI DOI 10.1007/BF02996744
[2] Chibata I, 1977, Adv Appl Microbiol, V22, P1, DOI 10.1016/S0065-2164(08)70158-8
[3] IMMOBILIZED ASPARTASE-CONTAINING MICROBIAL CELLS - PREPARATION AND ENZYMATIC PROPERTIES
CHIBATA, I
TOSA, T
SATO, T
[J]. APPLIED MICROBIOLOGY, 1974, 27 (05) : 878 - 885
[4] CHIBATA I, 1972, P INT FERMENT S FERM, V4, P383
[5] DISHE Z, 1951, J BIOL CHEM, V92, P583
[6] Mosbach K., 1976, METHOD ENZYMOL, V44
[7] REASON FOR HIGHER STABILITY OF ASPARTASE ACTIVITY OF IMMOBILIZED ESCHERICHIA-COLI-CELLS
TOSA, T
SATO, T
NISHIDA, Y
CHIBATA, I
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 483 (01) : 193 - 202
[8] TOSA T, 1966, ENZYMOLOGIA, V31, P214
[9] BASIC STUDIES FOR CONTINUOUS PRODUCTION OF L-ASPARTIC ACID BY IMMOBILIZED ESCHERICHIA-COLI-CELLS
TOSA, T
SATO, T
MORI, T
CHIBATA, I
[J]. APPLIED MICROBIOLOGY, 1974, 27 (05) : 886 - 889
[10] CHARACTERISTICS AND APPLICATIONS OF N-(OMEGA-AMINOHEXYL)-L-ASPARTIC ACID SEPHAROSE AS AN AFFINITY ADSORBENT
TOSA, T
SATO, T
SANO, R
YAMAMOTO, K
MATUO, Y
CHIBATA, I
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 334 (01) : 1 - 11

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