INVITRO FERTILIZATION AND DEVELOPMENT OF BOVINE OOCYTES MATURED IN SERUM-FREE MEDIUM

被引：93

作者：

SAEKI, K

HOSHI, M

LEIBFRIEDRUTLEDGE, ML

FIRST, NL

机构：

[1] SNOW BRAND MILK PROD CO LTD, LIFE SCI RES INST, ISHIBASHI, TOCHIGI 32905, JAPAN

[2] UNIV WISCONSIN, DEPT MEAT & ANIM SCI, MADISON, WI 53706 USA

来源：

BIOLOGY OF REPRODUCTION | 1991年 / 44卷 / 02期

关键词：

D O I：

10.1095/biolreprod44.2.256

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study was undertaken to investigate the effects of supplementation of serum (fetal calf serum), gonadotropins (LH, FSH, prolactin) and estradiol-17-beta (E2) to culture medium during in vitro maturation of bovine cumulus oocyte complexes on subsequent fertilization and development to the blastocyst stage in vitro. Serum supplementation during bovine oocyte maturation was not required but hormonal supplementation, gonadotropins (LH + FSH) and E2, enhanced the fertilizability and developmental ability of bovine oocytes matured in vitro. The addition of prolactin to maturation medium containing LH, FSH, and E2 did not further enhance frequencies of fertilization and development.

引用

页码：256 / 260

页数：5

共 28 条

[1] FACTORS AFFECTING SUCCESSFUL INVITRO FERTILIZATION OF BOVINE FOLLICULAR OOCYTES [J].