STABILITY OF IMMOBILIZED ALPHA-CHYMOTRYPSIN

被引：69

作者：

KAWAMURA, Y ^{[1
]}

NAKANISHI, K ^{[1
]}

MATSUNO, R ^{[1
]}

KAMIKUBO, T ^{[1
]}

机构：

[1] KYOTO UNIV, FAC AGR, DEPT FOOD SCI & TECHNOL, KYOTO 606, JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1981年 / 23卷 / 06期

关键词：

D O I：

10.1002/bit.260230605

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

引用

页码：1219 / 1236

页数：18

共 29 条

[1] CHEMICAL COUPLING OF ENZYMES TO CROSS-LINKED DEXTRAN (SEPHADEX) [J].

AXEN, R ;

PORATH, J .

NATURE, 1966, 210 (5034) :367-&

[2]

AXEN R, 1971, EUR J BIOCHEM, V18, P351

[3]

Berezin I V, 1976, Methods Enzymol, V44, P571

[4] CALCIUM-ION BINDING-SITE IN BOVINE CHYMOTRYPSIN-A [J].

BIRNBAUM, ER ;

ABBOTT, F ;

GOMEZ, JE ;

DARNALL, DW .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 179 (02) :469-476

[5] EQUILIBRIUM AND RATE CONSTANTS FOR INTERCONVERSION OF 2 CONFORMATIONS OF ALPHA-CHYMOTRYPSIN - EXISTENCE OF A CATALYTICALLY INACTIVE CONFORMATION AT NEUTRAL PH [J].

FERSHT, AR ;

REQUENA, Y .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 60 (02) :279-&

[6] SOME PROPERTIES OF A BACTERIAL PROTEINASE CHEMICALLY FIXED TO AGAROSE [J].

GABEL, D ;

HOFSTEN, BV .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 15 (03) :410-&

[7]

KLIBANOV AM, 1979, ANAL BIOCHEM, V93, P1

[8] STUDIES ON CONFORMATION OF SOLUBLE AND IMMOBILIZED ENZYMES USING DIFFERENTIAL SCANNING CALORIMETRY .2. SPECIFIC ACTIVITY AND THERMAL-STABILITY OF ENZYMES BOUND WEAKLY AND STRONGLY TO SEPHAROSE CL 4B [J].

KOCHSCHMIDT, AC ;

MOSBACH, K .

BIOCHEMISTRY, 1977, 16 (10) :2105-2109

[9] APPLICATION OF SPECTROPHOTOMETRIC METHODS TO DETERMINATION OF PROTEIN BOUND TO AGAROSE BEADS [J].

KOELSCH, R ;

LASCH, J ;

MARQUARDT, I ;

HANSON, H .

ANALYTICAL BIOCHEMISTRY, 1975, 66 (02) :556-567

[10] CONCERNING MECHANISM OF AUTOLYSIS OF ALPHA-CHYMOTRYPSIN [J].

KUMAR, S ;

HEIN, GE .

BIOCHEMISTRY, 1970, 9 (02) :291-&

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