DATA-BASE ANALYSIS OF PROTEIN EXPRESSION PATTERNS DURING T-CELL ONTOGENY AND ACTIVATION

被引:35
作者
HANASH, SM
STRAHLER, JR
CHAN, Y
KUICK, R
TEICHROEW, D
NEEL, JV
HAILAT, N
KEIM, DR
GRATIOTDEANS, J
UNGAR, D
MELHEM, R
ZHU, XX
ANDREWS, P
LOTTSPEICH, F
ECKERSKORN, C
CHU, E
ALI, I
FOX, DA
RICHARDSON, BC
TURKA, LA
机构
[1] UNIV MICHIGAN,SCH MED,DEPT HUMAN GENET,ANN ARBOR,MI 48104
[2] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,ANN ARBOR,MI 48104
[3] UNIV MICHIGAN,SCH MED,DEPT RHEUMATOL,ANN ARBOR,MI 48104
[4] UNIV MICHIGAN,SCH ENGN,DEPT IND & OPERAT ENGN,ANN ARBOR,MI 48109
[5] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY
关键词
PROTEIN DATA BASE; LYMPHOID PROLIFERATION; THYMIC MATURATION;
D O I
10.1073/pnas.90.8.3314
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have developed a data base of lymphoid proteins detectable by two-dimensional polyacrylamide gel electrophoresis. The data base contains two-dimensional patterns and derived information pertaining to polypeptide constituents of unstimulated and stimulated mature T cells and immature thymocytes, single-cell-derived T- and B-cell clones, leukemia cells, and lymphoid cell lines. Using this data base, we have compared the protein constituents of mature T cells and immature thymocytes before and after mitotic stimulation. A subset of polypeptides that are induced in mature T cells following mitotic stimulation were found to be constitutively expressed in immature thymocytes. Other polypeptides exhibited differences in their expression between mature and immature thymocytes in a manner unrelated to proliferation. The identity of several constitutively expressed or mitotically induced proteins in lymphoid cells was established by microsequencing. These initial findings point to significant differences in the molecular pathways leading to proliferation between mature and immature T cells. The construction of this database should facilitate further studies of lymphoid differentiation and function.
引用
收藏
页码:3314 / 3318
页数:5
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