MODIFIED RNA SEQUENCE POOLS FOR IN-VITRO SELECTION

被引：154

作者：

LIN, Y ^{[1
]}

QIU, Q ^{[1
]}

GILL, SC ^{[1
]}

JAYASENA, SD ^{[1
]}

机构：

[1] NEXAGEN INC,BOULDER,CO 80301

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 24期

关键词：

D O I：

10.1093/nar/22.24.5229

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the P'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.

引用

页码：5229 / 5234

页数：6

共 43 条

[1] 2'-FLUORO-2'-DEOXYNUCLEOSIDE AND 2'-AMINO-2'-DEOXYNUCLEOSIDE 5'-TRIPHOSPHATES AS SUBSTRATES FOR T7 RNA-POLYMERASE [J].