FLUORESCENCE CHARACTERISTICS OF SODIUM FLUORESCEIN IN PLASMA AND WHOLE-BLOOD

被引:55
作者
DELORI, FC
CASTANY, MA
WEBB, RH
机构
[1] Department of Retina Research, Eye Research Institute, Retina Foundation, Boston, MA 02114
关键词
angiography; blood; emission spectrum; excitation spectrum; sodium fluorescein;
D O I
10.1016/0014-4835(78)90020-9
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The present study encompasses in vitro measurements of the excitation and emission spectra of thin layers of plasma and whole blood containing sodium fluorescein. Measurements were performed using frontal illumination of the sample: that is, the emitted fluorescence was measured from the side of the sample which was illuminated by the excitation light beam, thereby simulating the conditions of fundus angiography. The influence of sodium fluorescein concentration, layer thickness, hematocrit, and oxygen saturation of the blood on the fluorescence characteristics was investigated. The experimental results were compared with a theoretical analysis of the effect of absorption by blood on the fluorescence of sodium fluorescein diluted in blood. © 1978.
引用
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页码:417 / 425
页数:9
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